Practical course in plant biotechnology (in Eng.)

English title:

Practical course in plant biotechnology

Course ID:

790327

ECTS credits:

4,5

Title in native language:
Practical course in plant biotechnology (in Eng.)

Term Semester:

Spring/Summer

Instruction language(s):

English

Course content:

Preparation of culture media, working under aseptic conditions; surface sterilization and preparation of the source isolate (tissue); initiation and passaging of callus cultures and suspension cultures, explants; isolation of protoplasts; exercises to study the influence of periphysis, cyclophysis and topophysis on the establishment of cultures, mediated transformation by means of Agrobacterium, GUS assay

Expected previous knowledge:

791111 Plant Biotechnology

Learning outcomes:

Students will be experienced in the practical implementation of the techniques of in-vitro culture and protein analyses:
+ know how to establish, maintain, propagate different samples of plant tissues in vitro
+ handle the transfer to glass house conditions
+ be able to induce somatic embryogenesis
+ carry out transformation experiments
+ verify the transgenic status of plant material
+ analyse independently plant (fruit ) samples for the presence of allergens

Teaching and learning methods:

Anmerkung: REMINDER:
To take part in the course, participants need a mark of 1 (Sehr Gut) or 2 (Gut) in the lecture VO Pflanzenbiotechnologie or an equivalent qualification.
The course will be held in the PBU Laboratory in MUG1 OH 6/02 on the following dates:
14. / 15. 3. from 13:00 - 17:00
18./19. 04. from 13:00 - 17:00
09./10. 05. from 13:00 - 17:00
23./24.05. from 13:00 - 17:00
30./31. 05. from 13:00 - 17:00
06/07. 06. from 13:00 - 17:00
13./14. 06. from 13:00 - 17:00
20./21.06. from 13:00 - 17:00

Exam method:

written protocol
active participation

Details URL:

https://online.boku.ac.at/BOKUonline/wbLv.wbShowLVDetail?pStpSpNr=293074

Scheduled dates:

http://online.boku.ac.at/BOKUonline/te_ortzeit.liste?corg=15660&clvnr=293074

Curriculum URL:

http://online.boku.ac.at/BOKUonline/wbLv.wbShowStellungInStp?pStpSpNr=293074