Gene technology for plant pathologists (in Eng.)

Students gain knowledge of:

- Basic genetic cloning methods including PCR, restriction enzymes, different plasmids and vectors etc.
- Plant transformation tools, selection markers and reporter genes.
- Examples of transgenic plants in the field.
- Resistance genes and host resistance.
- Technics of plant genome engineering including RNAi, ZFN, TALENs and CRISP/Cas9.
- Mechanisms of plant resistance against different pests and pathogens including induced resistance, systemic acquired resistance, induced systemic resistance, ETI and PTI etc.
- The role of secondary metabolites in the plant resistance.

Learning outcomes:

Upon completion of this course, the students will be able to:

- name and explain the principles of gene cloning including the PCR, digestion with restriction enzymes, ligation and selection of transformed bacteria using different systems
- name and explain the different plant resistant mechanisms including induced resistance, systemic acquired resistance, induced systemic resistance, ETI and PTI etc.
- name the different methods of plant genome engineering
- name and explain the role of different substances in the plant resistance such as phytoalexins, phytoanticipins and different secondary metabolites
- name the transformation tools such as Agrobacterium-mediated transformation and their role in plant-pathogen studies
- name examples of selection markers and reporter genes- name and describe examples of transgenic plants in the field and assess their advantages and disadvantages
- name and explain principles of resistance genes, classes of resistance genes and the location of the corresponding proteins
- name and explain principles of perception of pathogen effectors in plants and host resistance